“Ashiok” from the popular card game Magic: The Gathering is explicitly referred to as being nongendered. Though some depictions of the character include “he” as a pronoun, a lead designer from the company that makes the game has insisted on numerous occasions that the character is explicitly nongendered.[1] Even going so far as to write stories which avoid referring to Ashiok using gendered pronouns at all.[2] Ashiok's card can be found here.
Roswell, in the podcast The Adventure Zone, is an Earth Elemental made of living clay in a suit of armor, who talks via a small bird. Roswell is agender and uses they/them pronouns.[3]
Chaos Life by A. Stiffler and K. Copeland - A light-hearted, semi-autobiographical webcomic about the everyday idiosyncrasies of an agender person, their female partner, and their cats.
6ses by Kagome is a comic that features an agender protagonist.
*Eri the Cyborg[Dead link] by Ren is a comic that features an agender protagonist.
Biaggio, in the movie "The Kings of Summer," asserts that they don't see themself as "having a gender."
In the video game NiGHTS into Dreams the character "NiGHTS is neutral, and therefore has no gender. The impressions of the character with regards to gender are totally up to the player" according to Takashi Iizuka, the lead designer of the game.[4]
Bone Dance by Emma Bull. Character: the protagonist, Sparrow, is canonically described as "sexless" and "genderless." The exact details of their identity are a matter of debate (spoilers).
The entire race of Chronicoms from Marvel's Agents of S.H.I.E.L.D. are vehemently agender, though they are a race of extraterrestrial androids. They use gendered pronouns based on their current form.
In the indie romance visual novel My Cup of Coffee: Earl Grey Forever After, the protagonist can be male, female, or "gender free". The player can make all other characters genderfree as well, which results in the game using gender neutral language, ne/nem/nir pronouns, and the Mx title.